Journal of Pharmaceutical Research

D. Saraswathi ¹, G. P. Gigi ¹, Ajithadas Aruna ¹, A. Jerad Suresh ¹

Affiliations
1 Department of Pharmaceutical Chemistry, Madras Medical College, Chennai-600003, IN

Abstract

Two simple, sensitive and accurate spectrophotometric methods have been developed for the estimation of Acebrophylline in bulk and in pharmaceutical formulations (Method A&Method B). Method A is based on the formation of orange red colored chromgen with ferric chloride in the presence of 2,22-bipyridyl which obeys beer's law in the concentration range of 10-100 μg/ml exhibiting maximum absorption at 522nm. Method B is based on the diazotization of Acebrophylline with nitrous acid. The diazonium salt formed was then coupled with â-naphthol which shows maximum absorption at 456nm (yellow colored chromogen). It obeys Beer's law in the concentration ranging from 50-300 μg/ml. The methods were extended to capsule formulation and there was no interference from excipients and diluents. These methods have been statistically validated and are found to be precise and accurate.

Keywords

Acebrophylline (ACE), Ferric chloride, 2,22-Bipyridyl, Diazotization, α-Naphthol.

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V. Niraimathi ¹, V. Prema ¹, Ajithadas Aruna ¹, A. Jerad Suresh ¹

Affiliations
1 Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai - 600 003, Tamil Nadu, IN

Abstract

Four new, simple, sensitive and reproducible spectrophotometric methods have been developed for the estimation of cefditoren pivoxil in tablet dosage form. Method A involves the determination of cefditoren pivoxil by Standard absorbance method at 230nm and the Beer's concentration range was found to be 5-50 μg/mL. Method B and Method C involve the determination of cefditoren pivoxil by first derivative spectrophotometry and second derivative spectrophotometry respectively. The normal spectrum was derivatized to first and second order derivative spectrum and the linearity was found to lie within the Beer's range for cefditoren pivoxil. Method D involves the determination of cefditoren pivoxil by area under curve method and the linearity was established.

Keywords

Cefditoren Pivoxil(CP), Beer's Law, Standard Absorbance Method, Derivative Spectrophotometry, Area under Curve(AUC).

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A. Jerad Suresh ¹, A. Mohan Kumar ¹, T. Suyaraj ¹, D. Vinod ¹

Affiliations
1 Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai - 600003, IN

Abstract

A study was carried out using Reverse Phase High Performance Liquid Chromatography to determine the stability of four different brands of formulation (capsules) containing Amoxicillin and Dicloxacillin. Five batches of four different brands expiring in consecutive months (7^th, 8^th, 9^th, 10^th and 12^th month of 2010) were obtained and studied. During 5 months study, all the five batches were stored at room temperature (NMT 25°C). The 5 batches were assayed periodically at one month interval by Reverse Phase High Performance Liquid Chromatography method. The Dicloxacillin content in all the 5 batches steadily decreased over the months indicating that formulations of Amoxicillin and Dicloxacillin, which are available in the market are generally unstable. The Dicloxacillin content also reached 0% in some of these batches.

Keywords

HPLC, Amoxicillin, Dicloxacillin, Stability, Degradation Rate.

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S. Angayer Kanchana ¹, Aruna Ajithadas ¹, V. Niraimathi ¹, A. Jerad Suresh ¹

Affiliations
1 Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai-600 003, Tamil Nadu, IN

Abstract

Four new simple, accurate and precise spectrophotometric methods have been developed for simultaneous determination of telmisartan and amlodipine in pharmaceutical dosage form. Method A involves formation and solving of simultaneous equation using 299nm and 364nm as two wavelengths. Method B involves formation of Q-absorbance equation at 339nm (iso absorptive point) and at 299nm (λ_max of telmisartan). Method C involves first order derivative method for simultaneous estimation of these two drugs. Method D involves the AUC for first order derivative spectrum. Both the drugs obey the Beer's law in the range 5-50μg/mL for amlodipine and 5-40μg/mL for telmisartan. The results of analysis have been validated statistically and by recovery studies.

Keywords

Telmisartan (TEL), Amlodipine (AML), Simultaneous Equation, Q-Absorbance, Area Under Curve, Derivative Spectrophotometry.

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D. Saraswathi ¹, G. P. Gigi ¹, V. Niraimathi ¹, A. Jerad Suresh ¹

Affiliations
1 Department of Pharmaceutical Chemistry, Madras Medical College, Chennai-600 003, IN

Abstract

A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for the estimation of acebrophylline in capsule dosage form. A phenomenex Gemini C₁₈, 5μm column having 250×4.6mm i.d. in isocratic mode with mobile phase containing diammonium phosphate buffer (pH 4):methanol (60:40) was used. The flow rate was 1mL/minute and effluents were monitored at 273nm. The retention time was found to be 4.54 minutes. The linearity was in the range of 80-120 μg/mL. The proposed method was validated and successfully applied for the estimation of acebrophylline in capsule dosage form.

Keywords

Acebrophylline(ACE), RP-HPLC.

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A. Jerad Suresh ¹, A. Mohan Kumar ¹, S. Prabhu ¹

Affiliations
1 Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai-600 003, IN

Abstract

A study was carried out using LC-MASS Spectroscopy to determine the degradation products of formulation (capsules) containing Amoxicillin and Dicloxacillin. Previously an RP-HPLC method was performed to determine the stability of five batches of four different brands of formulation (capsules) containing Amoxicillin and Dicloxacillin which were expiring in consecutive months (7^th, 8^th, 9^th, 10^th and 12^th month of 2010). The Dicloxacillin content in all the five batches steadily decreased over the period of consecutive 5 month Assays (one month interval between each Assays), thereby determined the degradation product of Dicloxacillin by LC-MASS Spectroscopy.

Keywords

LC-MASS Spectroscopy, RP-HPLC, Amoxicillin, Dicloxacillin, Degradation Product.

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V. Niraimathi ¹, A. Jerad Suresh ¹, Lata Sriram ¹, T. Latha ¹

Affiliations
1 Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai-600 003, IN

Abstract

A novel series of azomethines of aryl oxazoles were synthesised and investigated for their antimicrobial activity against Gram positive and Gram negative bacteria using antibiotic diffusion method. The recorded data of zone of inhibition showed significant broad activity when compared with standard. The sensitivity of the Gram positive bacteria to the tested compounds was higher than that of Gram negative bacteria. The synthesised compounds were also screened for antifungal activity and observed that they had an inhibitory effect against the pathogenic fungi.

Keywords

Oxazoles, Azomethines, Antibacterial Activity, Antifungal Activity.

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V. Niraimathi ¹, A. Jerad Suresh ¹, Latha Sriram ¹, R. Kokila ¹

Affiliations
1 Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai-3, IN

Abstract

Aryl thiazoles and their azomethines attract a wide spread interest due to the diversified biological activities. Five new azomethines of aryl thiazole were synthesised and the structure were assigned according to their spectral data. These compounds were screened for their antibacterial activity against both Gram positive and Gram negative organisms. The recorded zone of inhibition showed significant broad spectrum activity when compared with standard ciprofloxacin. Antifungal screening indicated that all the synthesised compounds had inhibitory effect against the tested pathogenic fungi.

Keywords

Azomethines, Thiazoles, Antimicrobial Activity.

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V. Niraimathi ¹, A. Jerad Suresh ¹, K. Nanjappan ¹

Affiliations
1 Department of Pharmaceutical Chemistry, College of Pharmacy, Madras Medical College, Chennai–600 003, IN

Abstract

A reverse phase high performance liquid chromatographic method has been developed for the estimation of nisoldipine in tablet formulation. The separation was achieved by Phenomenex Gemini C₁₈ column (250×4.60mm, particle size 5ì) using water, acetonitrile and methanol (40:40:20) as mobile phase, at a flow rate of 1.5mL/min. Detection was carried out at 235 nm. Retention time of NIS was found to be 5.3min. The method has been validated for linearity, accuracy and precision. Linearity observed was in the range of 80-120μg/mL. The developed method was found to be accurate, precise, selective and rapid for estimation of nisoldipine in tablet dosage form.

Keywords

Nisoldipine, (NIS), RP-HPLC.

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